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xgen exome hyb panel v2  (Integrated DNA Technologies)


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    Integrated DNA Technologies xgen exome hyb panel v2
    Xgen Exome Hyb Panel V2, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 92/100, based on 102 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/xgen+exome+research+panel/xGen+Exome+Hyb+Panel+v2/custom%4010005151%4040108438
    Average 92 stars, based on 102 article reviews
    xgen exome hyb panel v2 - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Hybridization:

    Article Title: Exome sequencing identifies novel susceptibility genes and defines the contribution of coding variants to breast cancer risk
    Article Snippet: .. Afterwards, hybridization capture reagents for DNA libraries were used for overnight hybridization with the xGen Exome Research panel (Integrated DNA technologies, IDT), capture and amplification. .. Barcoded pooled libraries of 96 samples were sequenced on each lane of a NovaSeq 6000 S4 flowcell (Illumina) using NovaSeq Xp 4-Lane Kit (2×100bp).

    Article Title: Genomic characteristics of two breast malignant phyllodes tumors during pregnancy and lactation identified through whole-exome sequencing
    Article Snippet: Library preparations were performed with a KAPA Hyper Prep Kit (KAPA Biosystems). .. Target enrichment was performed using the xGen Exome Research Panel and Hybridization and Wash Reagents Kit (Integrated DNA Technology) according to the manufacturer’s protocol. .. Sequencing was performed on an Illumina HiSeq4000 platform using PE150 sequencing chemistry (Illumina).

    Amplification:

    Article Title: Exome sequencing identifies novel susceptibility genes and defines the contribution of coding variants to breast cancer risk
    Article Snippet: .. Afterwards, hybridization capture reagents for DNA libraries were used for overnight hybridization with the xGen Exome Research panel (Integrated DNA technologies, IDT), capture and amplification. .. Barcoded pooled libraries of 96 samples were sequenced on each lane of a NovaSeq 6000 S4 flowcell (Illumina) using NovaSeq Xp 4-Lane Kit (2×100bp).



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    The novel HNF4A variant affects the splicing of the gene. RT-PCR products obtained from Hek-293 cells transiently transfected with the different pSPL3 constructs. A band of 263 bp was detectable both in cells transfected with the empty vector (EV) and with the construct carrying the third HNF4A exon 3 and flanking <t>intronic</t> sequences with the c.391G>A variant (V). This is due to the skipping of the HNF4A exon induced by the presence of the substitution with combination of the two artificial exons as observed in cells expressing the empty vector. A band of 358 bp is detectable in cells expressing the wild-type minigene construct (WT) and corresponding to the correct splicing combining the exon 3 of the HNF4A gene with those provided by the splicing vector, as schematically represented in the middle panel. The splicing events were also checked by Sanger sequencing of the RT-PCR products as shown by the chromatograms reported in the right panel. 1kb and 100bp, molecular weight markers; -, PCR reaction negative control; SA and SD6, artificial exons provided by the pSPL3 vector.
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    The novel HNF4A variant affects the splicing of the gene. RT-PCR products obtained from Hek-293 cells transiently transfected with the different pSPL3 constructs. A band of 263 bp was detectable both in cells transfected with the empty vector (EV) and with the construct carrying the third HNF4A exon 3 and flanking intronic sequences with the c.391G>A variant (V). This is due to the skipping of the HNF4A exon induced by the presence of the substitution with combination of the two artificial exons as observed in cells expressing the empty vector. A band of 358 bp is detectable in cells expressing the wild-type minigene construct (WT) and corresponding to the correct splicing combining the exon 3 of the HNF4A gene with those provided by the splicing vector, as schematically represented in the middle panel. The splicing events were also checked by Sanger sequencing of the RT-PCR products as shown by the chromatograms reported in the right panel. 1kb and 100bp, molecular weight markers; -, PCR reaction negative control; SA and SD6, artificial exons provided by the pSPL3 vector.

    Journal: Frontiers in Medicine

    Article Title: Case Report: Beyond type 1 diabetes: a case of delayed MODY1 diagnosis and successful transition to sulfonylurea therapy

    doi: 10.3389/fmed.2025.1590935

    Figure Lengend Snippet: The novel HNF4A variant affects the splicing of the gene. RT-PCR products obtained from Hek-293 cells transiently transfected with the different pSPL3 constructs. A band of 263 bp was detectable both in cells transfected with the empty vector (EV) and with the construct carrying the third HNF4A exon 3 and flanking intronic sequences with the c.391G>A variant (V). This is due to the skipping of the HNF4A exon induced by the presence of the substitution with combination of the two artificial exons as observed in cells expressing the empty vector. A band of 358 bp is detectable in cells expressing the wild-type minigene construct (WT) and corresponding to the correct splicing combining the exon 3 of the HNF4A gene with those provided by the splicing vector, as schematically represented in the middle panel. The splicing events were also checked by Sanger sequencing of the RT-PCR products as shown by the chromatograms reported in the right panel. 1kb and 100bp, molecular weight markers; -, PCR reaction negative control; SA and SD6, artificial exons provided by the pSPL3 vector.

    Article Snippet: 50–100 ng of genomic DNA was used to sequencing selection of coding genomic regions and flanking intronic sequences using IDT xGen Exome Research Panel v2 enrichment kit (34 Mb, 19,433 genes) and Illumina technology (PE 2X150) on the Illumina platform Novaseq6000.

    Techniques: Variant Assay, Reverse Transcription Polymerase Chain Reaction, Transfection, Construct, Plasmid Preparation, Expressing, Sequencing, Molecular Weight, Negative Control